Abstract: The commonly used method of wing design optimization is firstly analyzed in this paper. Design optimization method of the wing profile airfoil distribution along the wing span is put forward, according to the requirments of special aerodynamic performance and wing configuration features for a high altitude long endurance aircraft. Hicks-Henne parameterization method is improved against the drawback of unsmooth tailing edge, which is subsequently utilized for the construction of wing dynamic mesh. B-splines function based threedimensional dynamic mesh generation method is proposed, as a substitute of commonly used linear interpolation based method. The customization and automation of data flow and work flow are realized by means of secondary development of commercial software Pointwise and Fluent, and all of the processes are integrated in iSIGHT platform. Finally, a wing optimization case for high altitude long endurance aircraft is studied using the aforementioned method, the optimization result shows that the endurance factor improves noticeably, indicates that the dynamic mesh based wing optimization method is an effective method.